All Boards >> Irritable Bowel Syndrome Research Library

View all threads Posts     Flat     Threaded

Gut Check - The bacteria in your intestines are welcome guests
      05/10/04 01:33 PM
HeatherAdministrator

Reged: 12/09/02
Posts: 7799
Loc: Seattle, WA

Gut Check - The bacteria in your intestines are welcome guests

John Travis

New York and London are famous for both their congestion and the diverse origins of their residents. But if you're looking for the ultimate teeming metropolis of immigrants, check out the large intestine. In people, some 500 to 1,000 kinds of bacteria reside in this part of the gastrointestinal (GI) tract, and these gut microbes outnumber all the cells in your body, perhaps by as much as a factor of 10.

"The density of this society is mind-boggling," says Jeffrey I. Gordon of Washington University School of Medicine in St. Louis.

It's a society overlooked by most microbiologists, who generally stick to the myriad bacteria that cause disease. Yet some scientists argue that it's shortsighted to ignore what they call the microflora living in our intestines.

"What these bacteria do definitely makes a very significant contribution to our health—or lack thereof," says Mark Schell of the University of Georgia in Athens, who studies an intestinal microbe called Bifodobacterium longum.

Shell and a few other researchers have recently begun to probe exactly what individual microbes do for or to the intestine.

Consider Bacteriodes thetaiotaomicron. Although not as well known, it's more than 1,000 times as abundant in the guts of people and mice as the extensively studied bacterium Escherichia coli. Some researchers have proposed that in return for a steady food supply, B. thetaiotaomicron breaks down indigestible complex carbohydrates into easily absorbed sugars and produces other substances, such as vitamins, that benefit its host.

There may be much more to this microbe-host relationship, however. About a decade ago, Gordon chose B. thetaiotaomicron as a prototypical germ for studying how microbes influence the GI tract. This bacterium normally becomes a predominant member of the intestinal community about the time an animal is weaned from its mother's milk. Gordon's research team has discovered that the microbe can turn on specific intestinal genes, promote the growth of blood vessels necessary for the gut's function, and trigger production of a chemical that may kill competing bacteria. Investigators are now asking just how much gut bacteria regulate the developing and adult human body.

"Bacteria do an awful lot for us and with us," says Gordon. "Most people's views of bacteria are of menacing, disease-promoting entities. Au contraire, I think that most of our encounters with bacteria are mutually beneficial, friendly, and part of our normal biology. . . . They've insinuated themselves into our biology and coevolved with us."

Sweet-talking germ

Perhaps the best way to understand the significance of intestinal microorganisms is to see what happens when an animal doesn't have them. During the past 50 years, researchers have created germfree mice and rats by delivering the animals by cesarean section into sterile environments and maintaining them there. "It's a very demanding technology," says Gordon. Scientists have generally used such germfree animals to study how particular pathogens cause diseases.

One of the most striking aspects of a germfree rodent is that it must consume about 30 percent more calories to maintain its body weight than a typical rodent does. Germfree animals are also unusually susceptible to infections, presumably because the microflora in a normal gut ward off foreign pathogens.

As a way to study animals hosting a simplified society of gut bacteria, Gordon and his colleagues have introduced B. thetaiotaomicron into germfree mice. Their first significant discovery was that the bacterium could change what sugars the intestine makes.

The surfaces of intestinal cells of typical mice are coated with complex sugars containing the simple sugar fucose and B. thetaiotaomicron consumes the fucose for energy. In germfree mice, however, fucose production ceases around the time of weaning.

If B. thetaiotaomicron colonizes a germfree mouse before weaning, however, normal fucose synthesis continues throughout life, the researchers found. Through a still undiscovered signal, the microbe apparently induces the intestinal cells to make one of its favorite foods. The bacterium even has a fucose sensor that informs it when this food source is scarce, according to Gordon and his colleagues.

The capacity of B. thetaiotaomicron to instruct intestinal cells to make fucose was just a hint of things to come. To get a more comprehensive picture of the bacterium's influence, Gordon's group turned to microchip-size devices, called DNA microarrays, that monitor the activity of thousands of genes at once (SN: 3/8/97, p. 144).

With such instruments, the scientists took a snapshot of the gene activity in the mouse intestine. By comparing tissue from germfree mice and mice hosting B. thetaiotaomicron, the team found that the presence of the bacterium significantly reduces or boosts the activity of about 100 of the approximately 25,000 rodent genes in the microarray survey.

Some of the intestinal genes triggered by the microbe help mammals absorb and metabolize sugars and fats, Gordon and his colleagues reported in 2001. Other activated genes fortify the cellular barrier that prevents bacteria, both dangerous and friendly, from sneaking out of the intestine into other tissues and the bloodstream. And yet other affected genes determine how the intestine detoxifies compounds and how the gut matures.

"We were amazed at the breadth of normal intestinal functions affected by a single microbe," says study coauthor Lora V. Hopper. Gordon adds, "It's difficult to anticipate the full range of host functions that might be manipulated by these microbes."

The genetic activity that the researchers didn't see in the bacteria-colonized mice was interesting, too. Even though the originally germfree mice had never encountered B. thetaiotaomicron before, there was no increase in activity of the genes underlying an immune or inflammatory response. That's a reflection of the microbe's still mysterious skill at convincing a host that it's a friendly visitor and not a danger, says Gordon.

Raising fences

Among the intestinal genes activated by B. thetaiotaomicron is one suspected to stimulate the growth of new blood vessels. The finding spurred Gordon's group to investigate the microbe's control over the system of blood vessels that runs through the GI tract. These blood vessels are crucial to a body's absorption of nutrients.

The researchers discovered that their germfree mice have a poorly formed network of the capillaries that normally supply the inner intestinal surface with its blood supply. This could partly explain the difficulty that germfree animals have absorbing nutrients.

The team also found that it could stimulate germfree mice to grow a normal network of intestinal capillaries by exposing the animals to either a full complement of microflora or just B. thetaiotaomicron. The investigators reported the finding in the Nov. 26, 2002 Proceedings of the National Academy of Sciences.

This is a vivid illustration that the physical development of the gut can depend on the microbes that normally inhabit animals, says Gordon. The researchers also found cells in the mouse gut that seems to work with the microbes to spur vessel growth.

In the small intestine, so-called Paneth cells normally secrete antimicrobial compounds (SN: 8/26/00, p. 135: Available to subscribers at http://www.sciencenews.org/20000826/fob8.asp). This keeps the intestine healthy by protecting other cells that continually replenish the gut lining. Gordon's team created germfree versions of mutant mice that lack Paneth cells and found that B. thetaiotaomicron couldn't trigger the maturation of blood vessels in such rodents. While most investigators have regarded Paneth cells simply as defenders against invading bacteria, it makes sense that these cells mediate interactions between a host and its natural microflora, says Gordon. "What better cell to respond to the presence or absence of a microbe?" he remarks.

The Paneth cell is at the heart of another microbe-intestine interaction uncovered recently by Gordon's group. One of the intestinal genes triggered in germfree mice by B. thetaiotaomicron encodes a protein called angiogenin 4 or Ang4. Cancer researchers are particularly interested in this protein, because they have evidence that it nourishes tumors by creating new blood vessels. Gordon's team suspected that Ang4 plays a role in the intestinal blood vessel maturation that they had documented earlier. Indeed, it turned out that Paneth cells make Ang4 and secrete it when they detect bacteria.

While the suspicion that Ang4 makes intestinal blood vessels has not yet been proven, it looks like the protein has a more certain role. It can kill several bacteria and fungi that cause diseases in mammals, Gordon, Hooper, and their colleagues report in the March Nature Immunology. In contrast, B. thetaiotaomicron and other common residents of the mouse intestines are largely resistant to Ang4.

"One interpretation of the interaction between host defense and the resident flora is that the resident bacteria that are resistant to Paneth-cell secretions stimulate these host-defense mechanisms to prevent competition by nonresident bacteria. The host in turn benefits by decreasing its exposure to potential pathogens," says Tomas Ganz of the University of California, Los Angeles in a commentary accompanying the March report.

Hooper agrees that the normal inhabitants of the gut may use Paneth cells and Ang4 to raise what she calls an "electric fence" to keep out competing microbes. Beyond fending off foreign pathogens, such fences may also keep typical intestinal microbes within the gut. "Anything can become a pathogen if it crosses the fence," she says.

Eating leftovers

Scientists have estimated that the hundreds of bacterial species within the human gut may together possess as many unique genes as a person does, and perhaps far more. "How much of our biology is dependent on metabolic traits encoded in the collective genomes of our microbial partners?" asks Gordon.

Investigators have begun to address that question. For example, Schell recently worked with scientists at the Nestlé Research Center in Lausanne, Switzerland, to unravel some of the genetic secrets of B. longum. This microbe typically colonizes the intestines of a newborn mammal, thrives during the breast-feeding period, and then subsides after weaning, when B. thetaiotaomicron and other bacteria take hold. Nestlé incorporates B. longum into some of its products, such as infant formulas and yogurts, to promote gastrointestinal health.

In the Oct. 29, 2002 Proceedings of the National Academy of Sciences, Schell and his colleagues unveiled the entire DNA sequence of B. longum and identified a large roster of genes for enzymes that break apart sugars and other edible substances. Some of these enzymes may degrade complex sugars found in breast milk, speculates Schell. Others, such as ones that apparently break down plant gums, may help the bacterium survive later in its host's life when B. longum is in the minority in the intestines.

The bacterium appears to break down substances that B. thetaiotaomicron and other Bacteriodes can't handle. "It seems to be more specialized for the leftovers of metabolism," says Schell. In a strategy similar to Gordon's, investigators at Nestlé are now using germfree mice to evaluate B. longum's impact on intestinal genes.

Gordon's team is drawing its own insights from the group's recent deciphering of B. thetaiotaomicron's genome. Among that microbe's nearly 4,800 genes, several hundred encode proteins that bind carbohydrates, enzymes that degrade bonds between sugars, or enzymes that create new sugars, the investigators reported in the March 28 Science. And the activity of many of these genes appears to be regulated by genes encoding molecules related to known environmental sensors, suggesting that the microbe can monitor the contents of the intestines and quickly deploy the molecular machinery needed for it to digest nutrients.

"This organism has a sweet tooth. It knows how to process carbohydrates," says Gordon.

Over the next 5 to 10 years, predicts Schell, researchers will decode the genomes of many more intestinal microbes. Investigators may also begin to address such issues as whether a person's diet changes his or her intestinal microflora. "I think the gut population of a vegetarian is clearly going to be different" from that of a meat eater, says Schell.

Gordon offers an even more provocative question: Do intestinal microbes influence a person's weight? "Over time, could relatively minor differences in the ability to extract nutrients in some individuals predispose them to obesity?" he asks.

The complicated nation of bacteria within our intestines is a "window into our biology and how we've evolved as a species," concludes Gordon.

http://www.sciencenews.org/articles/20030531/bob9.asp

--------------------
Heather is the Administrator of the IBS Message Boards. She is the author of Eating for IBS and The First Year: IBS, and the CEO of Heather's Tummy Care. Join her IBS Newsletter. Meet Heather on Facebook!

Print     Remind Me     Notify Moderator    

Entire thread
* Herbs, Probiotics, and Supplements
HeatherAdministrator
07/14/03 01:53 PM
* Western herbal medicines in the treatment of irritable bowel syndrome: A systematic review and meta-analysis
HeatherAdministrator
11/11/20 02:53 PM
* Medical cannabis: Promising uses supported by medical data
HeatherAdministrator
03/05/19 12:58 PM
* Glutamine supplements improve symptoms of postinfectious IBS-Diarrhea
HeatherAdministrator
08/22/18 02:35 PM
* Review article: the physiological effects and safety of peppermint oil and its efficacy in irritable bowel syndrome
HeatherAdministrator
01/26/18 12:06 PM
* Vitamin D deficiency in children and adults with IBS
HeatherAdministrator
04/07/17 03:18 PM
* Trial of aloe vera for irritable bowel syndrome
HeatherAdministrator
05/27/15 02:44 PM
* Aloe vera in Patients with Irritable Bowel Syndrome
HeatherAdministrator
05/27/15 02:39 PM
* Vitamins and IBS
HeatherAdministrator
03/11/14 02:10 PM
* IBS Quick Tip - Aloe, Senna, and Cascara for IBS?
HeatherAdministrator
09/10/13 11:20 AM
* IBS quick tip -taking a fiber supplements and medications
HeatherAdministrator
08/30/13 11:54 AM
* Fennel is an effective herbal drug for menstrual pain
HeatherAdministrator
08/21/13 05:59 PM
* Probiotic treatments relieve irritable bowel syndrome symptoms in recent study
HeatherAdministrator
01/18/13 02:38 PM
* Probiotics for GI Issues and Obesity - Problems May Take Root in Gut Flora
HeatherAdministrator
05/09/12 01:26 PM
* Probiotics May Prevent Antibiotic Diarrhea
HeatherAdministrator
05/09/12 12:38 PM
* Today Show Recommends Peppermint Oil Caps for IBS
HeatherAdministrator
12/02/11 03:58 PM
* Dr. Oz Recommends Peppermint Oil Capsules
HeatherAdministrator
11/23/11 02:32 PM
* Probiotic Pills Better for IBS than Yogurt
HeatherAdministrator
09/30/11 12:06 PM
* How Peppermint Helps to Relieve Irritable Bowel Syndrome
HeatherAdministrator
04/20/11 12:41 PM
* Zinc for acute diarrhea
HeatherAdministrator
02/10/11 10:18 AM
* Effect of ginger on gastric motility and symptoms of functional dyspepsia
HeatherAdministrator
02/10/11 10:16 AM
* Inulin can cause gastrointestinal discomfort
HeatherAdministrator
07/16/10 03:13 PM
* St. John's Wort Not Helpful For Irritable Bowel Syndrome
HeatherAdministrator
01/08/10 02:19 PM
* Systematic Review and Meta-Analysis: Probiotics in the treatment of Irritable Bowel Syndrome
HeatherAdministrator
02/18/09 02:16 PM
* Probiotic Yogurt Helps Reduce Physical Bloating
HeatherAdministrator
12/16/08 11:40 AM
* Peppermint oil, soluble fiber, and antispasmodics should be first-line treatments
HeatherAdministrator
12/03/08 11:37 AM
* Health Benefits of Probiotic Could Extend Beyond Gastrointestinal System
HeatherAdministrator
08/27/08 12:24 PM
* Acacia establishes prebiotic functionality in healthy human volunteers
HeatherAdministrator
06/19/08 11:54 AM
* Probiotics and prebiotics in irritable bowel syndrome
HeatherAdministrator
06/06/08 09:41 AM
* Gastrointestinal Microbiota in Irritable Bowel Syndrome
HeatherAdministrator
06/05/08 03:56 PM
* Efficacy of Probiotics in Irritable Bowel Syndrome
HeatherAdministrator
05/23/08 11:06 AM
* Probiotics in irritable bowel syndrome
HeatherAdministrator
01/18/08 12:32 PM
* Bacteria: a new player in gastrointestinal motility disorders-infections, bacterial overgrowth, and probiotics
HeatherAdministrator
11/07/07 02:49 PM
* Probiotic-prebiotic treatment for irritable bowel syndrome
HeatherAdministrator
08/28/07 01:29 PM
* Peppermint oil is effective in treating digestive disorders and other conditions including headaches
HeatherAdministrator
06/06/07 11:20 AM
* Probiotics and irritable bowel syndrome
HeatherAdministrator
05/11/07 02:25 PM
* Bacterial flora in irritable bowel syndrome
HeatherAdministrator
05/11/07 02:19 PM
* Peppermint oil capsules in the treatment of irritable bowel syndrome
HeatherAdministrator
04/28/07 10:36 AM
* Probiotics in women with IBS
HeatherAdministrator
02/04/07 04:17 PM
* Comparison of fennel and the drug mefenamic acid on pain intensity of menstrual cramps
HeatherAdministrator
12/01/06 02:27 PM
* Probiotics Effective for Treating Clostridium Difficile Disease and Antibiotic-Associated Diarrhea?
HeatherAdministrator
12/01/06 01:09 PM
* Review of Probiotics for IBD
HeatherAdministrator
07/17/06 02:10 PM
* A review of the bioactivity and potential health benefits of peppermint tea
HeatherAdministrator
07/17/06 12:01 PM
* Probiotics Help Chronic Constipation
HeatherAdministrator
07/05/06 05:40 PM
* Beneficial Bacteria Protect the Gut from Stress
HeatherAdministrator
05/29/06 02:25 PM
* Dietary fibre supplementation with psyllium or Acacia (gum arabic) reduced faecal incontinence
HeatherAdministrator
05/29/06 02:23 PM
* Probiotics and chronic disease
HeatherAdministrator
05/13/06 03:23 PM
* Peppermint Oil May Be "Drug of First Choice" for IBS
HeatherAdministrator
03/16/06 12:45 PM
* Herbal medicines for treatment of irritable bowel syndrome
HeatherAdministrator
02/18/06 12:54 PM
* Peppermint possesses antispasmodic properties
HeatherAdministrator
12/21/05 11:16 AM
* Pharmacological Basis for the Medicinal Use of Ginger in Gastrointestinal Disorders
HeatherAdministrator
12/19/05 04:50 PM
* Probiotic relieves irritable bowel syndrome
HeatherAdministrator
11/12/05 01:04 PM
* Melatonin May Reduce the Pain of Irritable Bowel Syndrome
HeatherAdministrator
10/28/05 11:53 AM
* A probiotic mixture alleviates symptoms in irritable bowel syndrome patients
HeatherAdministrator
09/22/05 04:26 PM
* Interactions Between Commensal Bacteria and Gut Sensorimotor Function
HeatherAdministrator
08/22/05 04:57 PM
* Prebiotic shown to increase bone mineral density
HeatherAdministrator
06/05/05 05:40 PM
* Fiber's New Role in Type 2 Diabetes
HeatherAdministrator
06/05/05 05:28 PM
* "Good" Bacteria Help Irritable Bowel Syndrome as much as Zelnorm and Lotronex
HeatherAdministrator
04/10/05 06:34 PM
* Complementary and Alternative Medicine Use by Patients With Gastrointestinal Diseases.
HeatherAdministrator
01/23/05 04:56 PM
* New evidence supports camomile tea's health benefits
HeatherAdministrator
01/07/05 05:02 PM
* Effectiveness of a symbiotic preparation in irritable bowel syndrome
HeatherAdministrator
11/08/04 04:44 PM
* Probiotic VSL#3 Could Relieve Some Irritable Bowel Abdominal Bloating
HeatherAdministrator
11/08/04 04:04 PM
* Probiotic Improves Postinfective Gut Dysfunction in Animal Model
HeatherAdministrator
10/11/04 04:30 PM
* Probiotics, dead or alive, can relieve gut disease
HeatherAdministrator
08/30/04 02:38 PM
* Prebiotics for improved gut health
HeatherAdministrator
07/11/04 03:15 PM
* Calcium Seems to Protect Against Colorectal Cancer
HeatherAdministrator
06/27/04 02:04 PM
* Safety of aloe a concern
HeatherAdministrator
05/25/04 03:26 PM
* Aloe, cascara sagrada, and senna laxtives can cause cathartic colon
HeatherAdministrator
05/25/04 03:24 PM
* Gut Check - The bacteria in your intestines are welcome guests
HeatherAdministrator
05/10/04 01:33 PM
* Probiotics and Prebiotics in Gastrointestinal Disorders
HeatherAdministrator
04/27/04 08:53 PM
* Yogurt Prevents Antibiotic-Associated Diarrhea
HeatherAdministrator
04/13/04 02:50 PM
* Bacteria Basics - Probiotics for IBS and IBD
HeatherAdministrator
03/30/04 01:56 PM
* Lactobacillus Reduces Irritable Bowel Bloating
HeatherAdministrator
12/01/03 06:06 PM
* Probiotic improves gastrointestinal symptoms in patients with chronic constipation
HeatherAdministrator
12/01/03 06:00 PM
* Aloe plant's IBS healing powers tested
HeatherAdministrator
11/18/03 03:42 PM
* Possible Drug Interactions with: Ginger
HeatherAdministrator
10/13/03 01:19 PM
* Hepatoprotective effect of Fennel essential oil
HeatherAdministrator
10/12/03 04:53 PM
* Fennel for the treatment of primary dysmenorrhea
HeatherAdministrator
10/12/03 04:51 PM
* Fennel, intestinal spasms, and infantile colic
HeatherAdministrator
10/12/03 04:48 PM
* Probiotics Significantly Reduce Symptoms of IBS, Ulcerative Colitis
HeatherAdministrator
07/22/03 09:41 PM
* Ginger: history and use for the prevention of nausea, dizziness, and vomiting
HeatherAdministrator
07/22/03 06:52 PM
* Efficacy of ginger for nausea and vomiting: a systematic review of randomized clinical trials.
HeatherAdministrator
07/22/03 06:50 PM
* Alteration of intestinal microflora and reduced IBS bloating and pain
HeatherAdministrator
07/18/03 12:01 PM
* Study on the efficacy of Lactobacillus in patients with irritable bowel syndrome.
HeatherAdministrator
07/18/03 11:42 AM
* Treatment for constipation-predominant IBS using Padma Lax, a Tibetan herbal formula
HeatherAdministrator
07/15/03 11:24 PM
* Ginger for nausea and vomiting in pregnancy
HeatherAdministrator
07/14/03 03:55 PM
* Peppermint-oil capsules in the treatment of IBS: a prospective, randomized trial
HeatherAdministrator
07/14/03 03:51 PM
* Efficacy of Peppermint oil in diarrhea predominant IBS
HeatherAdministrator
08/21/13 05:44 PM
* The effect of enteric-coated, delayed-release peppermint oil on irritable bowel syndrome.
HeatherAdministrator
08/21/13 05:46 PM
* Peppermint oil capsules for the treatment of IBS in children.
HeatherAdministrator
07/14/03 03:49 PM

Extra information
0 registered and 2 anonymous users are browsing this forum.

Moderator:  Heather 



Permissions
      You cannot post until you login
      You cannot reply until you login
      HTML is enabled
      UBBCode is enabled

Thread views: 1073059

Jump to

| Privacy statement Help for IBS Home

*
UBB.threads™ 6.2


HelpForIBS.com BBB Business Review